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A disposable poly(methylmethacrylate)‐based microfluidic module for protein identification by nanoelectrospray ionization‐tandem mass spectrometry
Author(s) -
Chen ShuHui,
Sung WangChou,
Lee GwoBin,
Lin ZhongYuan,
Chen PangWei,
Liao PaoChi
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<3972::aid-elps3972>3.0.co;2-6
Subject(s) - microfluidics , tandem mass spectrometry , mass spectrometry , chemistry , chromatography , tandem , capillary electrophoresis–mass spectrometry , fabrication , electrospray ionization , capillary action , analytical chemistry (journal) , nanotechnology , materials science , medicine , alternative medicine , pathology , composite material
The design, fabrication, and analytical use of a poly(methylmethacrylate) (PMMA)‐based microfluidic module for nanoelectrospray ionization‐tandem mass spectrometry (nano‐ESI‐MS/MS) were described. The microfluidic module can be mass‐produced at low costs and used as a disposable device to generate nano‐ESI‐MS/MS signals for protein identification from low amounts of protein samples. Compared with commercially available nanospray capillary tips, the module gave comparable signal quality and also offered advantages in convenience and easiness of operation, permitting repeated usage, and disposability.