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Miniaturization of capillary isoelectric focusing
Author(s) -
Wu XingZheng,
Sze Newman S.K.,
Pawliszyn Janusz
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<3968::aid-elps3968>3.0.co;2-9
Subject(s) - isoelectric focusing , miniaturization , myoglobin , capillary action , chromatography , chemistry , absorbance , analytical chemistry (journal) , materials science , nanotechnology , biochemistry , organic chemistry , composite material , enzyme
Miniaturization of whole‐column imaging capillary isoelectric focusing (CIEF) is discussed. A 1.2 cm capillary was used as a separation column for CIEF. The experimental results for the analysis of two p I markers and the protein myoglobin showed that good CIEF separation results could be obtained. Secondly, a light‐emitting diode (LED) was used as the light source for the whole‐column absorbance imaging detection. The focusing of both the p I markers and myoglobin were observed with the LED light source. The whole‐column imaging CIEF instrument was simplified and miniaturized by the use of the LED. Further developments are also discussed.