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High‐performance genetic analysis using microfabricated capillary array electrophoresis microplates
Author(s) -
Medintz Igor L.,
Paegel Brian M.,
Blazej Robert G.,
Emrich Charles A.,
Berti Lorenzo,
Scherer James R.,
Mathies Richard A.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co;2-0
Subject(s) - capillary electrophoresis , genotyping , analyte , electrophoresis , chromatography , chemistry , genotype , gene , biochemistry
This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (νCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high‐speed collection of sequencing and genotyping data from radially formatted νCAE devices. The concomitant development of a convenient energy‐transfer cassette labeling chemistry allows sensitive multicolor labeling of any DNA genotyping or sequencing analyte. High‐performance hereditary haemochromatosis and short tandem repeat genotyping assays are demonstrated on these devices along with rapid mitochondrial DNA sequence polymorphism analysis. Progress in supporting technology such as robotic fluid dispensing and batched data analysis is also presented. The ultimate goal is to develop a parallel analysis platform capable of integrated sample preparation and automated electrophoretic analysis with a throughput 10–100 times that of current technology.