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Investigation of the metabolism of substance P at the blood‐brain barrier using capillary electrophoresis with laser‐induced fluorescence detection
Author(s) -
Freed Anita L.,
Audus Kenneth L.,
Lunte Susan M.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:17<3778::aid-elps3778>3.0.co;2-e
Subject(s) - chemistry , chromatography , metabolism , microdialysis , capillary electrophoresis , fluorescence , microvessel , blood–brain barrier , laser capture microdissection , biochemistry , biology , central nervous system , immunology , endocrinology , physics , immunohistochemistry , extracellular , quantum mechanics , gene expression , gene
Substance P (SP) metabolism was investigated upon exposure to a monolayer of bovine brain microvessel endothelial cells (BBMECs), a cell culture model of the blood‐brain barrier. SP was incubated with the BBMECs and its metabolism was followed as a function of time over a 5‐h period. The resulting samples were derivatized with naphthalene‐2,3‐dicarboxaldehyde (NDA)/cyanide, separated, and detected using cyclodextrin‐modified electrokinetic chromatography with laser‐induced fluorescence detection (CDMEKC‐LIF). Upon exposure to the BBMEC monolayer, SP rapidly degraded to produce the N ‐terminal (1–9), (1–4) and (1–7) and C ‐terminal (2–11) and (3–11) fragments. These results were compared with those in an earlier report from our laboratory, where SP metabolism was investigated in vivo by microdialysis sampling in rat striatum.