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Nanoliter capillary electrochromatography columns based on collocated monolithic support structures molded in poly(dimethyl siloxane)
Author(s) -
Slentz Benjamin E.,
Penner Natalia A.,
Lugowska Emilia,
Regnier Fred
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:17<3736::aid-elps3736>3.0.co;2-y
Subject(s) - capillary electrochromatography , polydimethylsiloxane , miniaturization , monolithic hplc column , capillary action , siloxane , chromatography , materials science , molding (decorative) , theoretical plate , column (typography) , analytical chemistry (journal) , chemistry , polymer , nanotechnology , composite material , computer science , telecommunications , frame (networking)
Following current trends in miniaturization of analytical chemistry, an inexpensive disposable analytical tool in the form of a liquid chromatography column fabricated on a poly(dimethyl siloxane) (PDMS) chip was created. Ease of fabricating the chromatography column was demonstrated by molding collocated monolithic support structures (COMOSS) directly in the column. Positive photo‐resist, SPR 220, was used to create column structures on a negative relief master providing final channel dimensions of 2.7–5.2 νm wide by 10.0 νm deep, while monolithic dimensions were 9.8×9.8× 10.0 νm – 12.3×12.3×10.0 νm. The ability to separate biological samples such as peptides from a tryptic digest of fluorescein isothiocyanate labeled bovine serum albumin (FITC‐BSA) was shown. Separations in capillary electrochromatographic (CEC) mode were performed yielding column efficiencies of 4.0×10 5 plates/m.