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Spatial temperature gradient capillary electrophoresis for DNA mutation detection
Author(s) -
Zhu Liang,
Lee Hian Kee,
Lin Bingcheng,
Yeung Edward S.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:17<3683::aid-elps3683>3.0.co;2-b
Subject(s) - heteroduplex , capillary electrophoresis , temperature gradient , temperature gradient gel electrophoresis , melting temperature , capillary action , materials science , temperature control , mutation , dna , atmospheric temperature range , electrophoresis , chromatography , analytical chemistry (journal) , chemistry , thermodynamics , physics , biochemistry , 16s ribosomal rna , quantum mechanics , composite material , gene
A continuous spatial temperature gradient was established in capillary electrophoresis by using a simple temperature control device. The temperature profile along the capillary was predicted by theoretical calculations. A nearly linear spatial temperature gradient was established and applied to DNA mutation detection. By spanning a wide temperature range, it was possible to perform simultaneous heteroduplex analysis for various mutation types that have different melting temperatures.