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Survey of the distribution of red tide toxins (okadaic acid and dinophytoxin‐1) in the Dalian Bay sea area of China by micellar electrokinetic capillary chromatography
Author(s) -
Li Dazhi,
Sun Li,
Chen Zeling,
He Xinya,
Lin Bingcheng
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3583::aid-elps3583>3.0.co;2-i
Subject(s) - micellar electrokinetic chromatography , okadaic acid , bay , oceanography , china , red tide , chromatography , environmental science , chemistry , capillary electrophoresis , geography , geology , biochemistry , archaeology , phosphorylation , phosphatase
Two kinds of diarrhoetic shellfish toxins, okadaic acid (OA) and dinophytoxin‐1 (DTX‐1) were determined by micellar electrokinetic capillary chromatography (MEKC) with ultraviolet detection. A detection limit of 3.25 νg/mL for both of them was achieved. The UV absorbance of these toxins measured at 200 nm showed good linearity in the range of 6.25–200 νg/mL with R = 0.992 for OA and 0.997 for DTX‐1. Three kinds of shellfish ( Chlamys farreri, Mytilus edulis and Ruditaps philippinarum ) collected from eight locations (sampling in the intertidal zone) along the Dalian Bay sea area of China were surveyed in February and May of 2000. Results indicated that three kinds of shellfish were contaminated by OA and DTX‐1. Based on per gram of hepatopancreas in February, the contamination contents ranged from 0 to 1.26 νg for OA and from 0 to 1.82 νg for DTX‐1, and in May, the contents ranged from 0 to 1.45 νg for OA and 0 to 2.56 νg for DTX‐1. Among the eight locations, Hei Shi Jiao and Long Wang Tang were the most contaminated areas. Of the three kinds of shellfish, Mytilus edulis was the most significant species in accumulating OA and DTX‐1.