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Dynamics of single‐stranded DNA migration in denaturing polyacrylamide slab‐gel electrophoresis
Author(s) -
Djouadi Zahia,
Bottani Samuele,
Duval MarieAlix,
Siebert Rainer,
Tricoire Hrvé,
Valentin Luc
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3527::aid-elps3527>3.0.co;2-6
Subject(s) - electrophoresis , dna , fluorescence , slab , dynamics (music) , gel electrophoresis , laser induced fluorescence , resolution (logic) , biophysics , materials science , chemistry , optics , chemical physics , physics , computer science , biology , chromatography , biochemistry , artificial intelligence , geophysics , acoustics
Abstract We describe an original apparatus for the study of the dynamics of single stranded DNA migration. Four detectors based on laser‐induced fluorescence (LIF) are equidistantly placed on one migration lane, allowing repeated measurements of the same DNA band at different positions along migration. This article presents the characteristics and performances of this system and focuses on the data analysis, showing how the multiple detection scheme enables the study of band broadening and band resolution during a migration run. Our results suggest the existence of anomalous (nonthermal) diffusion of DNA molecules during the electrophoretic process.