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High‐throughput characterization for organic pollutants in environmental waters using a capillary electrophoresis chip
Author(s) -
Wakida Shinichi,
Chiba Atsushi,
Matsuda Toshio,
Fukushi Keiichi,
Nakanishi Hiroaki,
Wu Xiaoling,
Nagai Hidenori,
Kurosawa Shigeru,
Takeda Sahori
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3505::aid-elps3505>3.0.co;2-m
Subject(s) - capillary electrophoresis , throughput , pollutant , chromatography , pollution , chemistry , analytical chemistry (journal) , environmental chemistry , telecommunications , ecology , organic chemistry , computer science , wireless , biology
To evaluate organic pollution in water, we did preliminarily studies on high‐throughput characterization of organic pollution in water using microchip‐based capillary electrophoresis (CE) with laser‐induced fluorescence (LIF) detection. The applied voltage was investigated to control the gated valve injection and CE separation for conventional cross type microchips using a self‐made personal computer (PC)‐based controller as the voltage supply. We obtained high‐throughput data for the reproducible separation of fluorescein isothiocyanate (FITC)‐labeled river‐water samples using a zwitter‐ion based buffer solution to avoid adsorption of the labeled sample onto the channel of a microchip made from quartz glass. We used real samples from the Hino River that flows into Lake Biwa, from ten sampling points and obtained several reproducible peaks in different separation patterns for each sample within 2 min. We successfully demonstrated high‐throughput characterization of dissolved organic carbon (DOC) in environmental water using the microchip.