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Anomalous migration of hyaluronic acid oligomers in capillary electrophoresis: Correlation to susceptibility to hyaluronidase
Author(s) -
Kinoshita Mitsuhiro,
Okino Akiko,
Oda Yasuo,
Kakehi Kazuaki
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3458::aid-elps3458>3.0.co;2-0
Subject(s) - hyaluronic acid , hyaluronidase , capillary electrophoresis , chemistry , electrophoresis , fluorescence anisotropy , polymer , chromatography , polysaccharide , electrolyte , biochemistry , biophysics , enzyme , organic chemistry , biology , genetics , electrode , membrane
During high‐resolution capillary electrophoresis analysis in an electrolyte solution containing a neutral polymer, small oligomers of regularly arranged acidic polysaccharides such as hyaluronic acid and N ‐acetylneuraminic acid polymers showed reversal of the migration order. This anomalous migration was well correlated with their reported biological activity. In the present study, we analyzed hyaluronidase action on the purified hyaluronic acid oligomers using capillary electrophoresis and found that hydrolytic and transglycosylation actions by hyaluronidase were dependent on the molecular sizes of hyaluronic acid oligomers, and well correlated to their migration profiles. Furthermore, fluorescent polarization technique was employed for understanding the relationship between molecular size of hyaluronic acid oligomers and their electromigrations.