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Change of migration time and separation window accompanied by field‐enhanced sample stacking in capillary zone electrophoresis
Author(s) -
Hirokawa Takeshi,
Ikuta Natsuki,
Yoshiyama Tatsuya,
Okamoto Hikaru
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3444::aid-elps3444>3.0.co;2-q
Subject(s) - stacking , capillary electrophoresis , spark plug , electrolyte , capillary action , separation (statistics) , sample (material) , field (mathematics) , chemistry , flow (mathematics) , analytical chemistry (journal) , electrophoresis , plug flow , mechanics , materials science , chromatography , thermodynamics , physics , computer science , electrode , mathematics , organic chemistry , machine learning , pure mathematics , composite material
When field‐enhanced sample stacking was used in capillary zone electrophoresis (CZE) analysis of cations, the decrease of migration time and the reduction of separation window was observed with increase of sample plug length. A simple equation expressing the migration velocity in the stacking process was derived to explain the above phenomenon. From experiments and theoretical consideration, we confirmed that this effect was caused by the higher potential gradient and larger eletroosmotic flow (EOF) mobility at the sample plug than those at the supporting electrolyte. A mathematical model appropriate for the computer simulation of such a system was studied considering the experimental results, and it was concluded that electroosmotic velocity ( v eof ) should be introduced to the equation of continuity as a constant.