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Capillary electrophoresis method for the analysis of inorganic anions, organic acids, amino acids, nucleotides, carbohydrates and other anionic compounds
Author(s) -
Soga Tomoyoshi,
Imaizumi Motohiro
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3418::aid-elps3418>3.0.co;2-8
Subject(s) - capillary electrophoresis , chemistry , ethylenediaminetetraacetic acid , amino acid , electrophoresis , haloacetic acids , chromatography , electrolyte , nucleotide , inorganic ions , electrokinetic phenomena , organic chemistry , chelation , biochemistry , ion , chlorine , electrode , gene
A previously developed capillary zone electrophoresis (CZE) method with indirect UV detection for the simultaneous determination of inorganic and organic anions, amino acids and carbohydrates using 20 m M 2,6‐pyridinedicarboxylic acid (PDC) as the background electrolyte was extended to allow determination of 206 anions including those above – mentioned and physiological amino acids, nucleotides, aromatic acids, haloacetic acids, alcohols, phosphorylated saccharides, oxyhalides, metal oxoacids, metal‐ethylenediaminetetraacetic acid (EDTA) complexes, forensic anions, Good's buffers and herbicides. Every compound could be analyzed and their electrophoretic mobility determined simply by selecting detection wavelength. This method is simple and universal for anion analysis, and could be readily applied to the simultaneous determination of anionic compounds. In this work, it was used to identify and quantify important anions in sea urchin and sake.