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Analysis of DNA adducts bases by capillary electrophoresis with amperometric detection
Author(s) -
Inagaki Shinsuke,
Esaka Yukihiro,
Sako Magoichi,
Goto Masashi
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3408::aid-elps3408>3.0.co;2-a
Subject(s) - capillary electrophoresis , guanine , amperometry , chemistry , adduct , micellar electrokinetic chromatography , chromatography , dna , deoxyguanosine , dna adduct , biochemistry , organic chemistry , electrode , nucleotide , electrochemistry , gene
We have developed a method for the detection of DNA adducts by combining capillary electrophoresis (CE) with the specificity of amperometric detection. Guanine is the most easily damaged base of the four normal DNA bases and many adducts of guanine have been found in DNA. These guanine adducts are often electrochemically active, while the normal bases with the exception of guanine are not. Therefore, CE with amperometric detection will be a promising method to study DNA damage. The four normal deoxynucleosides and two damaged deoxnucleosides N 2 ‐ethyldeoxyguanosine ( N 2 ‐ethyl‐dG) and 8‐hydroxydeoxyguanosine (8‐OH‐dG), were completely separated by micellar electrokinetic chromatography (MEKC). Deoxyguanosine and the two damaged deoxynucleosides were identified using amperometric detection. The sensitivity of our system was comparable to that of UV detection. Analysis of DNA hydrolysis products was also performed briefly using this method.