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Separation of basic drug enantiomers by capillary zone electrophoresis using glucuronyl glucosyl β‐cyclodextrin as a chiral selector
Author(s) -
Matsunaga Hisami,
Haginaka Jun
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3382::aid-elps3382>3.0.co;2-#
Subject(s) - capillary electrophoresis , enantiomer , cyclodextrin , chemistry , chromatography , electrophoresis , capillary action , drug , stereochemistry , pharmacology , materials science , medicine , composite material
Separations of basic drug enantiomers have been investigated using glucuronyl glucosyl β‐cyclodextrin (GUG β‐CD) as a chiral selector in the background electrolyte by capillary zone electrophoresis. The effects of GUG β‐CD concentration and running buffer pH on the migration times and resolution of 16 basic drug enantiomers were precisely examined using a linear polyacrylamide‐coated capillary. High resolution of 16 basic drug enantiomers was generally attained with a running buffer pH 2.5 or 3.5 containing 10 m M GUG β‐CD. Next, we compared the chiral resolution abilities of GUG β‐CD with those of β‐CD and maltosyl β‐CD (G2 β‐CD). GUG β‐CD showed higher resolution for basic drug enantiomers tested than β‐CD and G2 β‐CD. This could be due to that hydrogen bonding or ionic interactions of uncharged and charged glucuronyl glucosyl groups of GUG β‐CD with an analyte could stabilize the inclusion complex.