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Capillary electrochromatography using fibers as stationary phases
Author(s) -
Jinno Kiyokatsu,
Watanabe Hiroshi,
Saito Yoshihiro,
Takeichi Tsutomu
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:16<3371::aid-elps3371>3.0.co;2-7
Subject(s) - capillary electrochromatography , capillary action , electrochromatography , packed bed , chromatography , fiber , kevlar , chemistry , analytical chemistry (journal) , materials science , composite material , composite number
Fiber‐packed capillary columns have been evaluated in chromatographic performance in capillary electrochromatography (CEC). The change of electroosmotic flow (EOF) velocity and selectivity using different kinds of fiber materials was examined. Although the EOF velocity among the different fiber packed columns was almost the same, retention of parabens was larger on the Kevlar®‐packed column than on the Zylon®‐packed one, and was larger on the as‐span‐type fiber‐packed column than on the high‐modulus‐type packed one. Using 200 νm ID × 5 cm Kevlar® packed column combined with a 100 νm ID × 20 cm precolumn capillary and a 530 νm ID × 45 cm postcolumn capillary, the separation of three parabens within 30 s was achieved. Other compounds were also separated in a few minutes by the fiber‐packed CEC method.