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Direct chiral resolution of malic acid in apple juice by ligand‐exchange capillary electrophoresis using copper(II)‐ L ‐tartaric acid as a chiral selector
Author(s) -
Kodama Shuji,
Yamamoto Atsushi,
Matsunaga Akinobu,
Soga Tomoyoshi,
Hayakawa Kazuichi
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:15<3286::aid-elps3286>3.0.co;2-j
Subject(s) - malic acid , tartrate , tartaric acid , chemistry , capillary electrophoresis , copper , resolution (logic) , ligand (biochemistry) , chromatography , carboxylate , electrophoresis , nuclear chemistry , inorganic chemistry , stereochemistry , organic chemistry , biochemistry , citric acid , receptor , artificial intelligence , computer science
Chiral resolution of native DL ‐malic acid was achieved by ligand‐exchange capillary electrophoresis using copper(II)‐ L ‐tartrate as a chiral selector. Factors affecting chiral resolution, migration time, and peak area of malic acid were studied. The running conditions for optimum separation of malic acid were found to be 1 m M copper(II) sulfate‐1 m M L ‐tartrate (pH 5.1) with an effective voltage of –20 kV at 30°C, using direct detection at 280 nm, and resolution ( R s ) of racemic malic acid was approximately 4. With this system, D ‐ and L ‐malic acids in apple juice were analyzed successfully.