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Enantioselective analysis of albendazole sulfoxide in cerebrospinal fluid by capillary electrophoresis
Author(s) -
Paias Fernanda O.,
Lanchote Vera L.,
Takayanagui Osvaldo M.,
Bonato Pierina Sueli
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200109)22:15<3263::aid-elps3263>3.0.co;2-4
Subject(s) - albendazole , enantiomer , chromatography , capillary electrophoresis , chemistry , sulfoxide , sulfone , anthelmintic , derivatization , mass spectrometry , stereochemistry , organic chemistry , medicine , surgery , veterinary medicine
Abstract Albendazole (ABZ) is a benzimidazole anthelmintic drug used in the treatment of neurocysticercosis. After oral administration, ABZ is rapidly oxidized to albendazole sulfoxide (ABZSO), which has an asymmetric sulfur center, and later to albendazole sulfone (ABZSO 2 ). ABZSO is the active metabolite responsible for the therapeutic effect of the drug. Previous studies have demonstrated pharmacokinetic differences between the two enantiomers, with the predominance of (+)‐ABZSO in human biological fluids. This article describes for the first time the enantioselective analysis of ABZSO in cerebrospinal fluid (CSF) using capillary electrophoresis. The samples were prepared by liquid‐liquid extraction using chloroform:isopropanol (8:2 v/v). The resolution of ABZSO enantiomers was obtained with a fused‐silica capillary (60 cm×75 νm ID) using 20 mmol/L Tris, pH 7.0, with 3.0% w/w sulfated β‐cyclodextrin as running buffer. The coefficient of variations and % relative error obtained for both within‐day and between‐days assays were lower than 15%. The method was linear over the concentration range of 100 to 2500 ng/mL for each enantiomer, indicating that it is suitable for the analysis of ABZSO enantiomers in CSF from patients medicated with ABZ.