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Identification of peroxisomal membrane proteins of Saccharomyces cerevisiae by mass spectrometry
Author(s) -
Schäfer Heike,
Nau Katja,
Sickmann Albert,
Erdmann Ralf,
Meyer Helmut E.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200108)22:14<2955::aid-elps2955>3.0.co;2-u
Subject(s) - mass spectrometry , peroxisome , chromatography , chemistry , protein mass spectrometry , peroxisomal disorder , electrospray ionization , membrane protein , membrane , biochemistry , gel electrophoresis , gene
The identification of peroxisomal membrane proteins is very important to understand the import mechanisms of substrates and proteins into these organelles and the pathogenesis of human peroxisomal disorders like the Zellweger Syndrom. Peroxisomal membrane proteins were identified after separation by gel electrophoresis, tryptic digestion and mass spectrometric analysis. Using matrix assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS) and nanoliquid chromatography‐electrospray ionization‐mass spectrometry (LC‐ESI‐MS), it was possible to identify 45 proteins of isolated yeast peroxisomal membranes.