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Direct electrochemical determination of pyruvate in human sweat by capillary zone electrophoresis
Author(s) -
Jin Mingliang,
Dong Qian,
Dong Rui,
Jin Wenrui
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200108)22:13<2793::aid-elps2793>3.0.co;2-a
Subject(s) - saturated calomel electrode , capillary electrophoresis , chromatography , analytical chemistry (journal) , chemistry , capillary action , electrophoresis , electrode , electrochemistry , detection limit , reference electrode , materials science , composite material
Capillary zone electrophoresis was employed for the determination of pyruvate in human sweat using electrochemical detection with a carbon fiber microdisk bundle electrode at a constant potential of 1.60 V vs. saturated calomel electrode. The optimum separation conditions are 3.6×10 –3 mol/L Na 2 HPO 4 ‐1.4×10 –3 mol/L NaH 2 PO 4 (pH 7.2) for the buffer solution, and 18 kV for the separation voltage. The limits of detection of pyruvate are 8.0×10 –6 mol/L or 24 fmol ( S/N = 3) for the injection voltage of 6 kV and the injection time of 10 s. The relative standard deviation is 2.0% for the migration time and 5.7% for the electrophoretic peak current. The method was applied to determining pyruvate in human sweat.