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Monitoring diclofenac sodium in single human erythrocytes introduced by electroporation using capillary zone electrophoresis with electrochemical detection
Author(s) -
Dong Qian,
Jin Wenrui
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200108)22:13<2786::aid-elps2786>3.0.co;2-v
Subject(s) - diclofenac sodium , capillary electrophoresis , chromatography , sodium , chemistry , detection limit , lysis , electroporation , electrophoresis , analytical chemistry (journal) , capillary action , calibration curve , materials science , biochemistry , organic chemistry , composite material , gene
A method for determination of the drug diclofenac sodium introduced into individual human erythrocytes by electroporation using capillary zone electrophoresis with electrochemical detection at a carbon fiber array microelectrode was developed. In this method, the whole cell was injected into the separation capillary by electromigration. Cell lysis was accomplished by injecting a plug of the separation buffer (1.25×10 –2 mol/L Na 2 B 4 O 7 –3.13×10 –3 mol/L NaOH). The optimum conditions of separation and detection were 20 kV for the separation voltage and 1.0 V for the detection potential. The concentration of diclofenac sodium in the single cells was quantified by a calibration curve. The mean concentration of diclofenac sodium introduced into the cell was 4.21 νmol/L. The relative standard deviation of the concentration of diclofenac sodium introduced into ten cells is 10%.