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On‐line identification of trans ‐ and cis‐ resveratrol by nonaqueous capillary electrophoresis/ fluorescence spectroscopy at 77 K
Author(s) -
Lin ChengHuang,
Chen YiHsiu
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200107)22:12<2574::aid-elps2574>3.0.co;2-m
Subject(s) - capillary electrophoresis , chromatography , chemistry , fluorescence spectroscopy , detection limit , electrophoresis , analytical chemistry (journal) , fluorescence , capillary action , spectroscopy , materials science , physics , quantum mechanics , composite material
This work presents a novel method for the accurate determining trans‐ and cis‐ resveratrol (3,5,4′‐trihydroxystilbene) by nonaqueous capillary electrophoresis/fluorescence spectroscopy at 77 K. The proposed method permits not only the separation of resveratrol isomers, but also ensures that on‐line spectra are readily distinguishable and unambiguously assigned. The experimental results also indicate that the effect of nonaqueous capillary electrophoresis buffer and low‐temperature technique increase the detection limit by more than 150‐fold.