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Separation of peptides utilizing ultrahigh‐temperature micellar electrokinetic chromatography
Author(s) -
Djordjevic Nebojsa M.,
Fitzpatrick Fiona,
Houdiere Fabrice
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200107)22:12<2398::aid-elps2398>3.0.co;2-6
Subject(s) - micellar electrokinetic chromatography , chromatography , electrokinetic phenomena , chemistry , resolution (logic) , capillary action , boiling , analytical chemistry (journal) , solvent , capillary electrophoresis , materials science , organic chemistry , computer science , composite material , artificial intelligence
The use of ultrahigh column temperatures, up to 110°C, in micellar electrokinetic capillary chromatography was investigated. The number of plates generated per unit time increased from 0.22 to 12.8 plates/s for separations at 15°C and 110°C, respectively. Ultrahigh‐temperature micellar electrokinetic capillary chromatography was used for the separation of cyclic undecapeptides (cyclosporins). A minimum resolution of 1.39 was calculated for a critical peak pair at 110°C, which is more than a 50% increase over resolution generated at 40°C. During a run time of more than 90 min at 110°C and at pH 9.3, no sample degradation or solvent boiling was observed.