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Time‐dependent expression and processing of a hypothetical protein of possible importance for regulation of the Chlamydia pneumoniae developmental cycle
Author(s) -
Vandahl Brian Berg,
Gevaert Kris,
Demol Hans,
Hoorelbeke Bart,
Holm Arne,
Vandekerckhove Jöel,
Christiansen Gunna,
Birkelund Svend
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:9<1697::aid-elps1697>3.0.co;2-d
Subject(s) - obligate , chlamydia , biology , pathogen , microbiology and biotechnology , protein expression , intracellular parasite , chlamydophila pneumoniae , human pathogen , bacteria , intracellular , chlamydiales , genetics , gene , ecology
Chlamydia pneumoniae is an obligate intracellular human pathogen infecting epithelial cells of the upper respiratory tract. It is a Gram‐negative bacteria and has a unique biphasic developmental cycle. In this study, we use two‐dimensional gel electrophoresis in combination with radioactive labeling to investigate time‐dependent expression and processing of C. pneumoniae proteins. We report on (i) the identification of a hypothetical protein which is expressed late in the developmental cycle and subsequently processed; we speculate that this protein may be of importance for the developmental cycle of Chlamydia; (ii) the identification of the major outer membrane protein in three different variants, which may all be present in vivo.