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Computer‐assisted evaluation of isoelectric focusing patterns in electrophoretic gels: Identification of smoothhounds (Mustelus mustelus, Mustelus asterias) and comparison with lower value shark species
Author(s) -
Re Pietro,
Colombo Marco M.,
Colombo Fabio,
Malandra Renato,
Biondi Pier A.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:8<1534::aid-elps1534>3.0.co;2-d
Subject(s) - biology , zoology , squalus acanthias , fishery , biochemistry
In this work, the most commercially important Selachian species, smoothhound (Mustelus mustelus) and starry smoothhound (Mustelus asterias) , have been identified by polyacrylamide isoelectric focusing (IEF‐PAGE), along with several shark species of minor commercial value. In Italy, these two smoothhound species are commonly subjected to fraudulent substitution with lesser valued sharks. After the electrophoretic runs, the band patterns of the two Mustelus species were compared with those of dogfish (Scyliorhinus canicula) , spurdog (Squalus acanthias) , blue shark (Prionace glauca) and black‐mouthed dogfish (Galeus melanostomus) , both visually and with gel analysis software. The actual isoelectric points were then submitted to cluster analysis to differentiate the single species, despite the possible occurrence of electrophoretic variations or protein polymorphism. Every shark showed species‐specific band patterns and could therefore be well differentiated, as confirmed by statistical analysis.