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Quantification of L ‐ascorbic acid and total ascorbic acid in fruits and spinach by capillary zone electrophoresis
Author(s) -
Liao Taching,
Jiang ChiiMing,
Wu MingChang,
Hwang JeanYu,
Chang HungMin
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:8<1484::aid-elps1484>3.0.co;2-9
Subject(s) - ascorbic acid , dehydroascorbic acid , spinach , capillary electrophoresis , chemistry , chromatography , detection limit , vitamin c , food science , biochemistry
A standard curve for the quantification of L ‐ascorbic acid (L‐AA) by capillary zone electrophoresis (CZE) was established, and the quantification of ascorbic acid and total ascorbic acid in fruits (lemon, Sunkist, and pineapple) and spinach were performed using D ‐isoascorbic acid ( D ‐IAA) as an internal standard. The minimum detection limits (MDLs) for L ‐AA and D ‐IAA were determined to be 1 and 2 νg/mL, respectively, at 265 nm. Dehydroascorbic acid (DHAA) in fruits and spinach was quantified in the presence of DL ‐homocysteine. The recoveries for L ‐AA in these juices were between 95 and 105%.