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Methods for the determination of binding constants by capillary electrophoresis
Author(s) -
Rundlett Kimber L.,
Armstrong Daniel W.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:7<1419::aid-elps1419>3.0.co;2-v
Subject(s) - capillary electrophoresis , analyte , dissociation constant , chemistry , micelle , dissociation (chemistry) , chromatography , biochemistry , receptor , aqueous solution
Apparent equilibrium constants for molecular association ( e.g., association constants, binding constants, dissociation constants, partition coefficients) can be determined with a variety of different capillary electrophoresis (CE) approaches. In many cases, the investigated association behavior is between a smaller molecule or ion ( i.e., the solute, drug, or analyte of interest) and a larger entity ( e.g., proteins, micelles, polymers, chiral selectors such as cyclodextrins, etc .). Each experimental approach has advantages and disadvantages. Frequently, it is the nature of the system being evaluated that determines the optimal experimental approach. Six different CE‐based techniques for evaluating binding constants are reviewed. Examples of each method, and recent references on its use are given.