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Enantioseparation of ofloxacin in urine by capillary electrokinetic chromatography using charged cyclodextrins as chiral selectors and assessment of enantioconversion
Author(s) -
de Boer Theo,
Mol Roelof,
de Zeeuw Rokus A.,
de Jong Gerhardus J.,
Ensing Kees
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:7<1413::aid-elps1413>3.0.co;2-u
Subject(s) - chromatography , capillary electrophoresis , chemistry , ofloxacin , micellar electrokinetic chromatography , urine , electrokinetic phenomena , cyclodextrin , capillary action , materials science , antibiotics , biochemistry , ciprofloxacin , composite material
A method was developed for the enantioseparation of ofloxacin, a member of the fluoroquinolones, using an anionic cyclodextrin‐derivative with or without combination with a neutral cyclodextrin‐derivative, as the chiral selector (s) in an electrokinetic chromatography system. The best results were obtained with 0.35 m M sulfated β‐cyclodextrin dissolved in a 50 m M phosphate buffer, pH 2.5, and at 15°C. Under these conditions, a resolution of 2 was readily achieved. Furthermore, under adequate separation conditions, studies were performed in order to assess possible in vitro and in vivo enantioconversion of levofloxacin. The current method allows detection of 2 μg R ‐(+)‐ofloxacine/mL diluted urine without the necessity of sample cleanup.