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Separation of 4‐dimethylamino‐6‐(4‐methoxy‐1‐naphthyl)‐1,3,5‐triazine‐2‐hydrazine derivatives of carbonyl compounds by reversed‐phase capillary electrochromatography
Author(s) -
Liu Zhen,
Zou Hanfa,
Ye Mingliang
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200105)22:7<1298::aid-elps1298>3.0.co;2-x
Subject(s) - capillary electrochromatography , chemistry , micellar electrokinetic chromatography , chromatography , reagent , elution , triazine , high performance liquid chromatography , hydrazine (antidepressant) , derivatization , electrochromatography , phase (matter) , reversed phase chromatography , capillary electrophoresis , organic chemistry
4‐Dimethylamino‐6‐(4‐methoxy‐1‐naphthyl)‐1,3,5‐triazine‐2‐hydrazine (DMNTH) is a novel derivatizing reagent specially designed for the determination of carbonyl compounds. In this work, we describe the separation of DMNTH‐derivatized carbonyl compounds by reversed‐phase capillary electrochromatography (CEC). After systematic investigations of the effects of experimental conditions viz. pH and concentration of buffer, type of stationary phase, injection volume of sample, organic modifier, and temperature, optimal conditions were found. The sample compounds, which were separated with gradient high performance liquid chromatography (HPLC), were separated by CEC under isocratic elution due to the high efficiency. Comparisons of separations by CEC and micellar electrokinetic chromatography (MEKC) were made.