Premium
New block‐copolymer thermoassociating matrices for DNA sequencing: Effect of molecular structure on rheology and resolution
Author(s) -
Sudor Jan,
Barbier Valessa,
Thirot Sylvie,
Godfrin Dominique,
Hourdet Dominique,
Millequant Michelle,
Blanchard Julien,
Viovy JeanLouis
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200102)22:4<720::aid-elps720>3.0.co;2-n
Subject(s) - lower critical solution temperature , copolymer , polymer , rheology , capillary electrophoresis , materials science , side chain , polymer chemistry , capillary action , viscosity , electrophoresis , chemical engineering , grafting , microstructure , chemistry , chromatography , composite material , engineering
A new family of matrices for DNA sequencing by capillary electrophoresis is presented. These matrices combine easy injection with high sieving performances, due to thermal switching between a low and a high viscosity state through a modest increase in temperature (˜20°C). They are constructed from a hydrophilic polymer backbone with grafted lower critical solution temperature (LCST) side chains. The comb‐like LCST copolymers are characterized in terms of size of the polymer backbone, the size of LCST side chains and the grafting densities. The dependance of rheological behavior and electrophoretic performance of these copolymers is correlated with their microstructure. Without complete optimization, a resolution of order 0.5, corresponding to a very reasonable limit for read length with current base calling softwares, could be achieved for segments around 800 bases differing by 1 base in less than one hour in a commercial ABI 310 apparatus.