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Analysis of metacycline by capillary electrophoresis
Author(s) -
Gil Eddy Castellanos,
Dehouck Pieter,
Van Schepdael Ann,
Roets Eugène,
Hoogmartens Jos
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200102)22:3<497::aid-elps497>3.0.co;2-h
Subject(s) - capillary electrophoresis , repeatability , chromatography , chemistry , electrolyte , capillary action , methanol , analytical chemistry (journal) , sodium carbonate , selectivity , electrophoresis , sodium , materials science , electrode , biochemistry , catalysis , organic chemistry , composite material
The development and validation of an optimized capillary electrophoresis method for the determination of metacycline in the presence of its related substances by capillary electrophoresis is shown. The influence of methanol as organic modifier, buffer pH, buffer concentration, capillary length, column temperature, Triton X‐100 and methyl‐β‐cyclodextrin was investigated. A central composite design was performed in order to optimize the method. The optimal separation conditions were: uncoated fused‐silica capillary (39 cm total length, 31 cm effective length, 50 μm ID); as background electrolyte a solution of 160 m M sodium carbonate and 1 m M EDTA (pH 10.35)/methanol (89:13 v/v); temperature, 15°C; voltage, 12 kV. The method showed good selectivity, repeatability, linearity, and sensitivity. The limits of detection and quantitation are 0.024% and 0.06%, respectively, relative to a 2.5 mg/mL solution. Six commercial samples were analyzed quantitatively.