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Fabrication and evaluation of a carbon‐based dual‐electrode detector for poly(dimethylsiloxane) electrophoresis chips
Author(s) -
Gawron Andrew J.,
Martin R. Scott,
Lunte Susan M.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200101)22:2<242::aid-elps242>3.0.co;2-w
Subject(s) - fabrication , electrode , detector , carbon fibers , materials science , dual (grammatical number) , electrophoresis , nanotechnology , optoelectronics , analytical chemistry (journal) , chromatography , chemistry , computer science , composite number , composite material , telecommunications , medicine , alternative medicine , pathology , art , literature
The first carbon‐based dual‐electrode detector for microchip capillary electrophoresis (CE) is described. The poly(dimethylsiloxane) (PDMS)‐based microchip CE devices were constructed by reversibly sealing a PDMS layer containing separation and injection channels to another PDMS layer containing carbon fiber working electrodes. End‐channel amperometric detection was employed and the performance of the chip was evaluated using catechol. The response was found to be linear between 1 and 600 μ M with an experimentally determined limit of detection (LOD) of 500 n M and a sensitivity of 30 pA/μ M . Collection efficiencies for catechol ranged from 36.0 to 43.7% at field strengths of 260—615 V/cm. The selectivity that can be gained with these devices is demonstrated by the first CE‐based dual‐electrode detection of a Cu(II) peptide complex. These devices illustrate the potential for a rugged and easily constructed microchip CE system with an integrated carbon‐based detector of similar scale.