Premium
Monitoring human serum transferrin by capillary zone electrophoresis with end‐column amperometric detection
Author(s) -
Dong Qian,
Yu Daiqing,
Ye Xiaoying,
Jin Wenrui
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200101)22:1<128::aid-elps128>3.0.co;2-l
Subject(s) - capillary electrophoresis , amperometry , transferrin , chromatography , chemistry , capillary action , electrophoresis , biochemistry , materials science , electrode , composite material , electrochemistry
Capillary zone electrophoresis was employed for the determination of human serum transferrin using end‐column amperometric detection with a carbon fiber microelectrode at a constant potential of 1.9 V vs. saturated calomel electrode (SCE). The optimum conditions of separation and detection are 7.5 × 10 −4 mol/L Tris‐3.44 × 10 −4 mol/L HCl for the buffer solution, 20 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, respectively. The limit of detection is 6.7 × 10 −8 mol/L or 440 amol (S/N = 2). The relative standard deviations are 0.67% for the migration time and 1.5% for the electrophoretic peak current. The method was applied to the determination of transferrin in human serum. The recovery is between 93—104%.