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Chiral analysis of baclofen by α‐cyclodextrin‐modified capillary electrophoresis and laser‐induced fluorescence detection
Author(s) -
Chiang MeiTsu,
Chang Sarah Y.,
Whang ChenWen
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200101)22:1<123::aid-elps123>3.0.co;2-4
Subject(s) - chromatography , chemistry , enantiomer , cyclodextrin , capillary electrophoresis , baclofen , detection limit , derivatization , laser induced fluorescence , fluorescence , high performance liquid chromatography , stereochemistry , biochemistry , physics , receptor , quantum mechanics , agonist
An enantioselective method for baclofen (4‐amino‐3‐ p ‐chlorophenylbutyric acid) based on capillary electrophoresis (CE) separation and laser‐induced fluorescence (LIF) detection has been developed. Naphthalene‐2,3‐dicarboxaldehyde (NDA) was used for precolumn derivatization of the nonfluorescent drug. α‐Cyclodextrin (α‐CD) was included in the buffer as a chiral selector for the separation of NDA‐labeled S ‐(+)‐ and R ‐(—)‐baclofen. Optimal resolution and detection were obtained with an electrophoretic buffer of 50m M sodium borate (pH9.5) containing 7m M α‐CD and a He‐Cd laser (λ ex = 442nm, λ em = 500nm). Combined with a simple cleanup procedure, this method can be applied to the analysis of baclofen enantiomers in human plasma. The relative standard deviation (RSD) values on peak areas of a plasma sample containing 1.0μ M racemic baclofen were 6.4 and 4.9% ( n = 8) for the S ‐(+)‐ and R ‐(—)‐enantiomer, respectively. The RSD value on migration times of both enantiomers was 0.5% ( n = 8). Calibration graphs for S ‐(+)‐ and R ‐(—)‐baclofen in plasma showed a good linearity ( r ≥0.999) in the concentration range of 0.1—2.0μ M . The limit of detection of baclofen in plasma was about 10ng/mL.