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Polymeric matrices for DNA sequencing by capillary electrophoresis
Author(s) -
Albarghouthi Methal N.,
Barron Annelise E.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200012)21:18<4096::aid-elps4096>3.0.co;2-w
Subject(s) - capillary electrophoresis , polymer , dna , dna sequencing , electrophoresis , polymer physics , gel electrophoresis of nucleic acids , chromatography , gel electrophoresis , chemistry , nanotechnology , materials science , organic chemistry , biochemistry
We review the wide range of polymeric materials that have been employed for DNA sequencing separations by capillary electrophoresis. Intensive research in the area has converged in showing that highly entangled solutions of hydrophilic, high molar mass polymers are required to achieve high DNA separation efficiency and long read length, system attributes that are particularly important for genomic sequencing. The extent of DNA‐polymer interactions, as well as the robustness of the entangled polymer network, greatly influence the performance of a given polymer matrix for DNA separation. Further fundamental research in the field of polymer physics and chemistry is needed to elucidate the specific mechanisms by which DNA is separated in dynamic, uncross‐linked polymer networks.