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Proteome analysis of activated murine B‐lymphocytes
Author(s) -
Frey Johann Rudolf,
Fountoulakis Michael,
Lefkovits Ivan
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200011)21:17<3730::aid-elps3730>3.0.co;2-5
Subject(s) - proteome , chemistry , computational biology , biology , microbiology and biotechnology , chromatography , biochemistry
Proteins extracted from murine B‐lymphocytes after in vitro stimulation by lipopolysaccharide were separated by two‐dimensional (2‐D) polyacrylamide gel electrophoresis and analyzed by matrix assisted laser desorption/ionization mass spectrometry. Structural information on the protein entities from 153 spots was obtained. Since many of these spots occur as members of spot families, a smaller number — 98 genes — was found to be coding for the identified spots. The elucidated proteins belong to groups of functional categories; we found 26 enzymes, 36 regulatory proteins, 15 chaperones, 15 structural proteins, 4 immunoglobulins, 1 ribosomal and 1 histone protein. A comparison between expected and observed molecular masses yields a good correlation for the majority of the compared spot entities. This set of proteins now identified in the context of a lymphocyte 2‐D gel pattern should advance further studies on lymphocyte functions.