Premium
Rapid determination of fungicides in fruit juices by micellar electrokinetic chromatography: Use of organic modifiers to enhance selectivity and on‐column high‐salt stacking to improve sensitivity
Author(s) -
Molina Manuel,
Silva Manuel
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200011)21:17<3625::aid-elps3625>3.0.co;2-k
Subject(s) - stacking , chromatography , chemistry , procymidone , micellar electrokinetic chromatography , captan , vinclozolin , carbendazim , myclobutanil , fungicide , detection limit , organic chemistry , botany , biology
A rapid, reliable method for the multiresidue analysis of eight commonly used fungicides by micellar electrokinetic chromatography (MEKC) was developed. Excellent separation of the eight fungicides (carbendazim, metalaxyl, captan, procymidone, folpet, captafol, vinclozolin and iprodione) is achieved within about 10 min by using optimized electrophoretic conditions that include the addition of a mixture of organic modifiers to the running buffer for improved resolution. The sensitivity of the method is enhanced by using an enrichment step that involves on‐column high‐salt stacking. Limits of detection in the microgram‐per‐liter region and relative standard deviations from 2.1 to 5.9% are thus obtained for the fungicides without detracting from peak resolution. These results reveal that the high‐salt stacking method provides highly improved sensitivity and enables highly flexible adjustment of the selectivity of the separation method. Also, the method surpasses other stacking alternatives used in MEKC and affords routine analyses of fruit juice containing fungicides at trace levels following a straightforward sample treatment. The robustness of the high‐salt stacking method as demonstrated in this work makes MEKC methods involving stacking procedures an attractive choice for routine analyses.