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Enantioseparation of dihydropyridine derivatives by means of neutral and negatively charged β‐cyclodextrin derivatives using capillary electrophoresis
Author(s) -
Christians Thorsten,
Holzgrabe Ulrike
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(200011)21:17<3609::aid-elps3609>3.0.co;2-0
Subject(s) - chemistry , capillary electrophoresis , enantiomer , cyclodextrin , chromatography , ether , methanol , stereochemistry , organic chemistry
Employing capillary electrophoresis, the racemates of 29 acidic, neutral and basic dihydropyridines (DHPs) were separated by means of neutral and negatively charged cyclodextrins (CDs). Whereas the enantiomers of the acidic DHPs could be resolved with neutral CDs, mostly α‐ and β‐CD, the enantiomers of the neutral DHPs were only baseline‐separated using the sulfobutyl ether‐substituted β‐CD. Working in reversed polarity mode (detector at the anode) improved the peak shape and the resolution of the enantiomers. The racemates of the DHP bearing a secondary or tertiary amine function in the side chain at position 3 could be separated by using either the neutral γ‐CD or negatively charged CDs. The poor peak shape found with anionic CDs could be improved by the addition of methanol. The combination of γ‐CD and sulfated β‐CD allowed the detection of the minor enantiomer of lercanidipine ( 24 ) at less than 1% w/w.