Premium
Biochemical analysis of a caveolae‐enriched plasma membrane fraction from rat liver
Author(s) -
Calvo Mario,
Enrich Carlos
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20001001)21:16<3386::aid-elps3386>3.0.co;2-l
Subject(s) - caveolae , immunoelectron microscopy , membrane , caveolin , biology , cell membrane , membrane protein , chemistry , microbiology and biotechnology , biochemistry , antibody , immunology
We isolated and characterized a subcellular fraction derived from the blood‐sinusoidal plasma membrane of hepatocytes enriched in caveolin and containing several of the molecular components described to be present in caveolae isolated from other cell types. A morphological study by electron microscopy revealed that it was composed of caveolae‐attached membrane profiles. Immunoelectron microscopy of isolated fraction showed the specific labeling of internal caveolae membranes with anti‐caveolin antibody. Finally, one‐ and two‐dimensional electrophoresis and Western blotting were used for the biochemical analysis of this new rat liver plasma membrane fraction. From the biochemical and the morphological characterization, we conclude that the caveolae‐enriched plasma membrane fraction is a plasma membrane fraction, which originates from specialized regions of the sinusoidal plasma membrane, enriched in caveolae.