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Proteomics meets cell biology: The establishment of subcellular proteomes
Author(s) -
Jung Eva,
Heller Manfred,
Sanchez JeanCharles,
Hochstrasser Denis F.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20001001)21:16<3369::aid-elps3369>3.0.co;2-7
Subject(s) - proteome , proteomics , organelle , subcellular localization , biology , computational biology , protein subcellular localization prediction , microbiology and biotechnology , cytoplasm , bioinformatics , biochemistry , gene
Proteome research aims to unravel the biological complexity encoded by the genome. Due to the complexity of higher eukaryotic cells, single‐step characterization of a proteome is likely to be difficult to achieve. However, advantage can be taken of the macromolecular architecture of a cell, e.g. , subcellular compartments, organelles, macromolecular structures and multiprotein complexes, to establish subcellular proteomes. This review highlights recent developments in this area of proteomics, namely the establishment of two‐dimensional electrophoresis (2‐DE) reference maps of subcellular compartments and organelles as well as the characterization of macromolecular structures and multiprotein complexes using a proteomics approach.