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On‐column derivatization and analysis of amino acids, peptides, and alkylamines by anhydrides using capillary electrophoresis
Author(s) -
Zhang Ying,
Gomez Frank A.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000901)21:15<3305::aid-elps3305>3.0.co;2-1
Subject(s) - derivatization , chemistry , capillary electrophoresis , chromatography , phthalic anhydride , acetic anhydride , electrophoresis , yield (engineering) , capillary action , reagent , organic chemistry , high performance liquid chromatography , catalysis , materials science , metallurgy , composite material
This work demonstrates the use of an in‐capillary procedure for derivatization of amino acids, peptides, and alkylamines by anhydrides using capillary electrophoresis (CE). Migrating in an uncoated fused‐silica capillary, plugs of substrate and anhydride are injected separately and electrophoresed. Differential transport velocities permit the separate zones to penetrate each other under an applied field, thereby facilitating reaction. In initial experiments the extent of reaction between tryptophan and acetic anhydride was examined and product amounts quantitated by CE. In separate experiments a series of amino acids and peptides were injected into the capillary and reacted with phthalic anhydride on‐column to yield the phthalic derivatized species. Finally, on‐column derivatization of alkylamines with phthalic anhydride was investigated and electrophoretic mobility related to molecular weight of the derivatized amines. These procedures illustrate the use of the capillary as a microreactor in the facile synthesis of derivatized molecules and ease of quantitation of reaction products under conditions of electrophoresis.