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Electrophoretic studies of polygalacturonate oligomers and their interactions with metal ions
Author(s) -
Wiedmer Susanne K.,
Cassely Aaron,
Hong Mingfang,
Novotny Milos V.,
Riekkola MarjaLiisa
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000901)21:15<3212::aid-elps3212>3.0.co;2-n
Subject(s) - capillary electrophoresis , chemistry , derivatization , polyacrylamide , electrophoresis , metal ions in aqueous solution , fluorescence , chromatography , ion , polymer , metal , magnesium , analytical chemistry (journal) , polymer chemistry , mass spectrometry , organic chemistry , physics , quantum mechanics
Polygalacturonic acid, a linear homopolysaccharide, was investigated by capillary electrophoresis (CE) using linear polyacrylamide‐coated capillaries and laser‐induced fluorescence (LIF) detection. A successful separation of its fluorescently labeled oligomers was achieved through sieving in polyacrylamide entangled matrices. The reaction conditions for the derivatization of polygalacturonic acid were optimized. In studying the interactions between polygalacturonic acid and various metal ions, the end‐label, free‐solution electrophoretic (ELFSE) technique, developed earlier in our laboratory (Sudor, J., Novotny, M. V., Anal. Chem. 1995, 67 , 4205—4209) was found preferable to the sieving method. ELFSE is fast and convenient in that no polymer solutions are needed for the separation. The investigation showed that for the moderately large oligomers, the strongest binding occurred with calcium and cadmium ions, while the smallest interaction was observed with magnesium ions.