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Enantiomeric separation by capillary electrochromatography II. Chiral separation of dansyl amino acids and phenoxy acid herbicides on sulfonated silica having surface‐bound hydroxypropyl‐β‐cyclodextrin
Author(s) -
Zhang Minquan,
El Rassi Ziad
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000901)21:15<3135::aid-elps3135>3.0.co;2-7
Subject(s) - capillary electrochromatography , enantiomer , chemistry , cyclodextrin , electrochromatography , chromatography , phase (matter) , amino acid , silica gel , capillary electrophoresis , organic chemistry , biochemistry
A chiral silica‐based stationary phase having surface‐bound hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD) with a relatively strong electroosmotic flow (EOF) was introduced for enantioseparation by capillary electrochromatography (CEC). The stationary phase contained a hydrophilic sulfonated sublayer to which a chiral top layer of HP‐β‐CD was immobilized. While the sulfonated sublayer was to provide a relatively strong EOF, the top HP‐β‐CD was to confer the desired chiral recognition towards enantiomeric solutes. This HP‐β‐CD sulfonated silica (CDSS) stationary phase proved useful for the rapid separation of anionic enantiomers such as dansyl amino acids and phenoxy acid herbicides. The effects of the organic modifier content, pH, and ionic strength of the mobile phase on enantioseparation were investigated. Under the optimized separation conditions, ten dansyl amino acids and six phenoxy acid herbicides were enantioseparated with a resolution greater than unity.