Premium
Polymer solutions as a pseudostationary phase for capillary electrochromatographic separation of DNA diastereomers
Author(s) -
Gilar Martin,
Belenky Alexei,
Cohen Aharon S.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2999::aid-elps2999>3.0.co;2-1
Subject(s) - diastereomer , chemistry , capillary electrophoresis , polymer , selectivity , formamide , chromatography , polyethylene glycol , polymer chemistry , chemical engineering , organic chemistry , engineering , catalysis
The solutions of linear polymers traditionally used for DNA separation have been employed for the capillary electrophoresis (CE) of diastereomers of chemically modified DNA. The selectivity of diastereomeric separation of the phosphorothioate (PS) and 2'‐ O ‐methylated (2‐OMe) PS oligonucleotides depends on the nature of the polymer additive in the CE background electrolyte. The selectivity of separation for different polymers increases in the line: linear polyacrylamide < polyethylene glycol < polyvinyl pyrrolidone. The separation of oligomer diastereomers was shown to be primarily based on the hydrophobic interaction with the polymer network that acts as a pseudostationary phase. While lowering the temperature resulted in improved separation, the addition of organic modifiers such as formamide, methanol or acetonitrile counteracts the solute adsorption on the polymer network, and decreases the selectivity of DNA diastereoseparation. The effect of molecular mass and concentration of the polymer on the separation selectivity was investigated.