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Analysis of acyclovir by high performance capillary electrophoresis with on‐column amperometric detection
Author(s) -
Zhang Shusheng,
Yuan Zhuobin,
Liu Hongxia,
Zou Hong,
Xiong Hui,
Wu Yangjie
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2995::aid-elps2995>3.0.co;2-p
Subject(s) - detection limit , amperometry , capillary electrophoresis , chromatography , chemistry , linear range , calibration curve , analytical chemistry (journal) , electrode , electrochemistry
The separation of acyclovir (ACV) by high performance capillary electrophoresis (HPCE) with on‐column amperometric detection using α‐amino‐5‐mercapto‐3,4‐dithiazole (AMD) as internal standard is described. The calibration line was linear in the range of 0.5—20 mg/L of ACV. The detection limit was 0.15 mg/L of ACV. Its recovery ranged from 98 to 101% with relative standard deviations (RSDs) from 1.9 to 3.2% ( n = 5). This method was successfully used for determining ACV in some pharmaceuticals and human urine. Comparable results with HPCE with ultraviolet (UV) detection and amperometric detection were obtained.