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Genomic clustering of the Trypanosoma cruzi nonlong terminal L1Tc retrotransposon with defined interspersed repeated DNA elements
Author(s) -
Olivares Monica,
Thomas Maria del Carmen,
LópezBarajas Almudena,
Requena José María,
GarcíaPérez José Luis,
Angel Sergio,
Alonso Carlos,
López Manuel Carlos
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2973::aid-elps2973>3.0.co;2-4
Subject(s) - retrotransposon , biology , genome , interspersed repeat , genetics , long terminal repeat , genomic dna , repeated sequence , retroposon , direct repeat , southern blot , tandem repeat , genomic library , dna , human genome , transposable element , gene , base sequence
We have analyzed the genomic distribution and organization of the long interspersed nucleotide element (LINE) L1Tc, a nonlong terminal repeat (LTR) retrotransposon of Trypanosoma cruzi . The results indicate that the L1Tc element is dispersed along the parasite genome and that in some regions it is organized in tandem repeats. The data allowed us to define the existence of short direct‐repeated sequences flanking the genomic L1Tc elements. Relevant is the finding that the LINE L1Tc is located in genomic regions rich in short interspersed nucleotide elements (SINE)‐like sequences. In particular, the L1Tc element is found associated to E13‐related sequences, redefined in this work and renamed RS13Tc, and to a newly described RS1Tc sequence. The RS1Tc sequence is present, per haploid genome, in about 3200 copies. Northern blot analysis showed that the RS1Tc is being transcribed into RNAs of different sizes. The analysis of the chromosomal distribution of these elements in various strains of T. cruzi suggested that this type of clustering might be a common feature of the genome of these parasites.