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Head column field‐amplified stacking from the flow: Stabilization of the sample plug position by using backpressure
Author(s) -
Kuldvee Ruth,
Kuban Petr,
Vunder Kadri,
Kaljurand Mihkel
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2879::aid-elps2879>3.0.co;2-b
Subject(s) - capillary electrophoresis , stacking , spark plug , sample (material) , capillary action , analyte , chromatography , analytical chemistry (journal) , chemistry , detection limit , flow (mathematics) , materials science , mechanics , physics , thermodynamics , organic chemistry , composite material
Head column‐field amplified sample stacking (HC‐FASS) is one of the most powerful concentration techniques in capillary electrophoresis. In the present work we demonstrate that a laboratory‐designed pneumatic sampler not only enables us to easily perform HC‐FASS from stagnant sample solutions but also permits us to carry out HC‐FASS from sample stream, which creates an opportunity to lower the detection limit of analytes further. The influence of stacking time in case of the flowing sample on peak efficiencies and resolution is discussed. It is demonstrated that the sample plug length can be kept constant by monitoring the current in the capillary, which controls a feedback system based on backpressure at the capillary outlet.