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Determination of some antirheumatics by capillary isotachophoresis
Author(s) -
Hercegová Andrea,
Sádecká Jana,
Polonský Jozef
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2842::aid-elps2842>3.0.co;2-m
Subject(s) - isotachophoresis , chemistry , chromatography , naproxen , ketoprofen , detection limit , ibuprofen , tris , hydrochloric acid , hydroxymethyl , electrolyte , inorganic chemistry , biochemistry , stereochemistry , pharmacology , medicine , alternative medicine , electrode , pathology
Isotachophoresis (ITP) was applied for the determination of some antirheumatic drugs (fenoprofen, naproxen, ibuprofen, and ketoprofen) in human serum. The leading electrolyte contained hydrochloric acid (10 mmol·L −1 ), creatinine (pH 4.5) and methylhydroxyethyl cellulose (0.1%). The terminating electrolyte was 2‐( N ‐morpholino)ethanesulfonic acid (10 mmol·L −1 ) adjusted with tris(hydroxymethyl)aminomethane to pH 6.9. The ITP separations were carried out in column‐coupling configuration of the separation unit provided with a preseparation column of 160 × 0.8 mm inner diameter (ID) and analytical column of 160 × 0.3 mm ID. The limit of detection for ibuprofen, fenoprofen, and naproxen in serum by direct sampling was 0.008, 0.005 and 0.004 mmol·L −1 . The limit of detection for ketoprofen in serum after ethanol precipitation was 0.001 mmol·L −1 .