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On‐line preconcentration methods for capillary electrophoresis
Author(s) -
Osbourn Damon M.,
Weiss David J.,
Lunte Craig E.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000801)21:14<2768::aid-elps2768>3.0.co;2-p
Subject(s) - stacking , capillary electrophoresis , isotachophoresis , chromatography , analyte , capillary action , chemistry , detection limit , capillary electrochromatography , electrokinetic phenomena , analytical chemistry (journal) , electrophoresis , sample preparation , micellar electrokinetic chromatography , materials science , electrolyte , organic chemistry , electrode , composite material
The limits of detection (LOD) for capillary electrophoresis (CE) are constrained by the dimensions of the capillary. For example, the small volume of the capillary limits the total volume of sample that can be injected into the capillary. In addition, the reduced pathlength hinders common optical detection methods such as UV detection. Many different techniques have been developed to improve the LOD for CE. In general these techniques are designed to compress analyte bands within the capillary, thereby increasing the volume of sample that can be injected without loss of CE efficiency. This on‐line sample preconcentration, generally referred to as stacking, is based on either the manipulation of differences in the electrophoretic mobility of analytes at the boundary of two buffers with differing resistivities or the partitioning of analytes into a stationary or pseudostationary phase. This article will discuss a number of different techniques, including field‐amplified sample stacking, large‐volume sample stacking, pH‐mediated sample stacking, on‐column isotachophoresis, chromatographic preconcentration, sample stacking for micellar electrokinetic chromatography, and sweeping.