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Global analysis of gene expression in cells of the immune system II. Cell‐free translation products and high‐density filter hybridization data
Author(s) -
Frey Johann Rudolf,
Nguyen Catherine,
Houlgatte Rémi,
Jordan Bertrand,
Auffray Charles,
Fol Bruno,
Kuhn Lotte,
Meyer Stefan,
Hartmann KlausUlrich,
Coleclough Christopher,
Kettman John R.,
Lefkovits Ivan
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:13<2694::aid-elps2694>3.0.co;2-e
Subject(s) - clone (java method) , biology , gene , computational biology , gene expression , complementary dna , translation (biology) , genetics , microbiology and biotechnology , messenger rna
We have developed an experimental system for linking information on cell‐free transcription and translation products from cDNA clones with data obtained from hybridization signals from complex probes. The work described in this paper consists of two distinct processes, one being the construction of a system of clonal addresses and the other the identification of expressed genes involved in the studied processes. We describe the use of this system to identify genes involved in thymus development. Complex probes from fetal thymuses (GD15, 17 and newborn) of Balb/c mice were used to identify genes, which are up‐ or downregulated during the process of differentiation. The full set of information is available in the Clone‐base of the Basel Institute for Immunology and will be retrievable from the website of the collaborating laboratories.