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Global analysis of gene expression in cells of the immune system I. Analytical limitations in obtaining sequence information on polypeptides in two‐dimensional gel spots
Author(s) -
Lefkovits Ivan,
Kettman John R.,
Frey Johann Rudolf
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:13<2688::aid-elps2688>3.0.co;2-t
Subject(s) - computational biology , proteomics , identification (biology) , complementary dna , cdna library , genomics , biology , genome , gene , computer science , data mining , genetics , botany
We have outlined various aspects and limitations of the collective analysis of protein species of a cell (lymphocyte). We have indicated research directions that, in to our opinion, deserve more attention. We have evaluated mainly the approach used in our laboratory and we recognize that a bulk of important research on the interface of proteomics and genomics remains to be dealt with. It is of great value that we can proceed in our quest by trial and error. But as much as the human genome initiative was not implemented by trial and error, but by formulating new technological approaches, we hope that our approach can be incorporated in the mainstream of proteomics. We need several integrating research directions, some of which are outlined in this communication, namely the use of ordered cDNA libraries, cell‐free expression systems, high density filter hybridization, identification of two‐dimensional (2‐D) gel spots in terms of their amino acid composition through biosynthetic labeling and identification of restriction sites in the corresponding coding sequences. In the accompanying paper the cDNA ordered library approach will be described in some detail.